e coli dna polymerase i (New England Biolabs)
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e coli dna polymerase i
E Coli Dna Polymerase I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 10189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e+coli+dna+polymerase/DNA+Polymerase+I/pmc12926610-373-25-30
Average 99 stars, based on 10189 article reviews
E Coli Dna Polymerase I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 10189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e+coli+dna+polymerase/DNA+Polymerase+I/pmc12926610-373-25-30
Average 99 stars, based on 10189 article reviews
e coli dna polymerase i - by Bioz Stars,
2026-10
99/100 stars
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other:Article Title: Macrophage MERTK restrains GRAMD1A-driven mitochondrial oxysterol trafficking to limit colitis. Article Snippet: PCR products were detected by 2% agarose gel electrophoresis, and the PCR products were recovered by gel-cutting with an AxyPrep DNA gel recovery kit (Axygen). Article Title: Macrophage MERTK restrains GRAMD1A-driven mitochondrial oxysterol trafficking to limit colitis Article Snippet: Poly(A) RNA was purified from 1 μg total RNA using Dynabeads Oligo(dT) (Thermo Fisher) through two rounds of purification, followed by fragmentation with the Purification:Article Title: Kinsenoside Targets IDH1 to Restore Microglial Immune-Metabolic Homeostasis for Alzheimer's Disease Therapy. Article Snippet: RNA integrity was verified by Agilent 2100 Bioanalyzer (RIN > 7.0) and denaturing agarose gel electrophoresis. .. Poly(A) RNA (1 μg total RNA input) was purified with Dynabeads Oligo (dT) 25 (Thermo Fisher) via two rounds of enrichment, then fragmented using Magnesium RNA Fragmentation Module (NEB) at 94◦C for 5–7 min. Cleaved RNA was reverse-transcribed to cDNA with SuperScript II Reverse Transcriptase (Invitrogen), followed by synthesis of U-labeled second-stranded DNAs using Article Title: Sfrp5 protects acute myocardial infarction by inhibiting macrophage foam cell formation and apoptosis via modulation of macrophage polarization and p38-MAPK/JNK/NF-κB pathways. Article Snippet: Polyadenylated mRNA was isolated from 5 μg of total RNA using two rounds of purification with Dynabeads Oligo(dT) (Thermo Fisher Scientific, USA). .. Purified mRNA was fragmented using divalent cations at elevated temperature (Magnesium RNA Jo ur na l P re -p ro of Fragmentation Module, New England Biolabs, USA) by incubating at 94°C for 5-7 min. First-strand cDNA was synthesized using SuperScript II Reverse Transcriptase (Invitrogen, USA), followed by second-strand synthesis incorporating dUTP using Reverse Transcription:Article Title: Kinsenoside Targets IDH1 to Restore Microglial Immune-Metabolic Homeostasis for Alzheimer's Disease Therapy. Article Snippet: RNA integrity was verified by Agilent 2100 Bioanalyzer (RIN > 7.0) and denaturing agarose gel electrophoresis. .. Poly(A) RNA (1 μg total RNA input) was purified with Dynabeads Oligo (dT) 25 (Thermo Fisher) via two rounds of enrichment, then fragmented using Magnesium RNA Fragmentation Module (NEB) at 94◦C for 5–7 min. Cleaved RNA was reverse-transcribed to cDNA with SuperScript II Reverse Transcriptase (Invitrogen), followed by synthesis of U-labeled second-stranded DNAs using Article Title: Integrated transcriptome and single-cell sequencing analysis identify blood-pancreas shared lncRNA biomarkers in new-onset T2DM. Article Snippet: Subsequent RNA fragmentation (NEB,USA) generated 200–300nt fragments through magnesium-catalyzed hydrolysis at 94°C. .. First-strand cDNA synthesis was performed using SuperScriptTM II Reverse Transcriptase (Thermofisher, USA), followed by second-strand synthesis with dUTP incorporation via Article Title: Clec7a promotes hippocampal microglial activation in rats with status epilepticus via inducing the TLR4/MyD88/NF-κB signaling pathway. Article Snippet: Microglial polarization imbalance between pro-inflammatory M1 and anti-inflammatory M2 phenotypes is a key mechanism in epilepsy-related neuroinflammation.. This study explores the role of C-type lectin domain containing 7A (Clec7a) in M1 microglial polarization in epilepsy.. An AAV-shClec7a was injected intra-hippocampally into Sprague-Dawley rats prior to induction of status epilepticus (SE) using lithium-pilocarpine. Article Title: Engineered Polylactic Acid (PLA) Microcapsules for Spatiotemporally Coupled Delivery and Synergistically Enhanced Dual Immunity Article Snippet: Poly(A) RNA was extracted from 1 μg of total RNA with Dynabeads Oligo dT25-61005 (Thermo Fisher, Waltham, CA, USA) undergoing two purification rounds. .. Then, the poly(A) RNA was divided into small fragments with the help of the Magnesium RNA Fragmentation Module (NEB, Ipswich, MA, USA) at 94 ◦C for 5 to 7 min. Next, SuperScriptTM II Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA) was employed to reverse-transcribe the cleaved RNA fragments into cDNA, which was subsequently used to synthesize U-labeled second-stranded DNAs with Article Title: Sfrp5 protects acute myocardial infarction by inhibiting macrophage foam cell formation and apoptosis via modulation of macrophage polarization and p38-MAPK/JNK/NF-κB pathways. Article Snippet: Polyadenylated mRNA was isolated from 5 μg of total RNA using two rounds of purification with Dynabeads Oligo(dT) (Thermo Fisher Scientific, USA). .. Purified mRNA was fragmented using divalent cations at elevated temperature (Magnesium RNA Jo ur na l P re -p ro of Fragmentation Module, New England Biolabs, USA) by incubating at 94°C for 5-7 min. First-strand cDNA was synthesized using SuperScript II Reverse Transcriptase (Invitrogen, USA), followed by second-strand synthesis incorporating dUTP using Article Title: Integrated transcriptome and single-cell sequencing analysis identify blood-pancreas shared lncRNA biomarkers in new-onset T2DM Article Snippet: Subsequent RNA fragmentation (NEB,USA) generated 200–300nt fragments through magnesium-catalyzed hydrolysis at 94°C. .. First-strand cDNA synthesis was performed using SuperScriptTM II Reverse Transcriptase (Thermofisher, USA), followed by second-strand synthesis with dUTP incorporation via cDNA Synthesis:Article Title: Integrated transcriptome and single-cell sequencing analysis identify blood-pancreas shared lncRNA biomarkers in new-onset T2DM. Article Snippet: Subsequent RNA fragmentation (NEB,USA) generated 200–300nt fragments through magnesium-catalyzed hydrolysis at 94°C. .. First-strand cDNA synthesis was performed using SuperScriptTM II Reverse Transcriptase (Thermofisher, USA), followed by second-strand synthesis with dUTP incorporation via Article Title: Integrated transcriptome and single-cell sequencing analysis identify blood-pancreas shared lncRNA biomarkers in new-onset T2DM Article Snippet: Subsequent RNA fragmentation (NEB,USA) generated 200–300nt fragments through magnesium-catalyzed hydrolysis at 94°C. .. First-strand cDNA synthesis was performed using SuperScriptTM II Reverse Transcriptase (Thermofisher, USA), followed by second-strand synthesis with dUTP incorporation via Control:Article Title: Clec7a promotes hippocampal microglial activation in rats with status epilepticus via inducing the TLR4/MyD88/NF-κB signaling pathway. Article Snippet: Microglial polarization imbalance between pro-inflammatory M1 and anti-inflammatory M2 phenotypes is a key mechanism in epilepsy-related neuroinflammation.. This study explores the role of C-type lectin domain containing 7A (Clec7a) in M1 microglial polarization in epilepsy.. An AAV-shClec7a was injected intra-hippocampally into Sprague-Dawley rats prior to induction of status epilepticus (SE) using lithium-pilocarpine. Synthesized:Article Title: Sfrp5 protects acute myocardial infarction by inhibiting macrophage foam cell formation and apoptosis via modulation of macrophage polarization and p38-MAPK/JNK/NF-κB pathways. Article Snippet: Polyadenylated mRNA was isolated from 5 μg of total RNA using two rounds of purification with Dynabeads Oligo(dT) (Thermo Fisher Scientific, USA). .. Purified mRNA was fragmented using divalent cations at elevated temperature (Magnesium RNA Jo ur na l P re -p ro of Fragmentation Module, New England Biolabs, USA) by incubating at 94°C for 5-7 min. First-strand cDNA was synthesized using SuperScript II Reverse Transcriptase (Invitrogen, USA), followed by second-strand synthesis incorporating dUTP using |